How was recombinant Factor VIIa developed?
  The development of recombinant Factor VIIa was undertaken to alleviate some of the problems associated with the use of plasma-derived Factor VIIa, such as limited supply and possible viral contamination. Multiple steps were involved in the development of this recombinant protein. First, the human gene for Factor VII, located on chromosome 13, was isolated from a liver gene library. A standard amplification procedure was used to generate multiple copies of the human Factor VII (hFVII) gene. The gene was then transfected into a baby hamster kidney (BHK) cell line. A master cell bank of the transfected cell line that secretes Factor VII in a single-chain form into the culture medium was then established.
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